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ccl1  (R&D Systems)


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    Structured Review

    R&D Systems ccl1
    Ccl1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+ccl1/Recombinant+Human+CCL1%2FI-309+Protein/pm41345127-329-14-16
    Average 94 stars, based on 10 article reviews
    ccl1 - by Bioz Stars, 2026-09
    94/100 stars

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    Recombinant:

    Article Title: STARTRAC analyses of scRNAseq data from tumor models reveal T cell dynamics and therapeutic targets
    Article Snippet: .. Recombinant human CCL1 (R&D Systems) was prepared at suboptimal concentration of 100 pM and added to the bottom Transwell chambers at 100 μl per well. ..

    Article Title: Biological characterization of ligands targeting the human CC chemokine receptor 8 (CCR8) reveals the biased signaling properties of small molecule agonists.
    Article Snippet: .. Recombinant human CCL1 (hCCL1, cat# 272-I), human CCL18 (hCCL18, cat# 394-PA-010), human CCL8 (hCCL8, cat#281-CP-050) and vMIP-I (cat# 600-VA-025; hereafter referred to as vCCL1) chemokines were purchased from R&D systems. .. AlexaFluor647-labelled hCCL1 (hCCL1AF647) was obtained from Almac (United Kingdom, cat# CAF-07).

    Article Title: Tumor cell entry into the lymph node is controlled by CCL1 chemokine expressed by lymph node lymphatic sinuses
    Article Snippet: .. Recombinant human CCL1, mouse CCL1, and viral MCV-type II chemokine-like protein MC148 were purchased from R&D Systems. .. We also used E. coli LPS (serotype 0127:B8; Sigma-Aldrich), recombinant human and mouse TNF (PeproTech), IL-1β (R&D Systems), FITC (Sigma-Aldrich), pertussis toxin (List Biological), cholera toxin (Sigma-Aldrich), Alexa Flour 594 phalloidin (Life Technologies), and Hoechst bisbenzimide (Sigma-Aldrich).

    Article Title: Tumor-Associated Macrophages Induce Migration of Renal Cell Carcinoma Cells via Activation of the CCL20-CCR6 Axis
    Article Snippet: .. Recombinant human CCL1 (272-I), CCL3 (270- LD), CCL4 (8228-MB), and CCL5 (278-RN) were purchased from R&D Systems (Minneapolis, MN, USA). ..

    Article Title: Ovine skin-recirculating γδ T cells express IFN-γ and IL-17 and exit tissue independently of CCR7
    Article Snippet: Briefly, whole lymph was collected 0–12h prior to the assay and incubated in RPMI containing 0.5% bovine serum albumin (Invitrogen) for 1 h. 5–10 × 10 5 cells in 100 μl were added to 5-μm-pore-sized, 24-well tissue culture inserts (Corning costar). .. Recombinant mouse CCL2, CCL17, CCL20, CCL21, and CXCL9 and recombinant human CCL1, CCL4, CCL20, and CCL28 (all from R&D Systems) were titrated in triplicates, and cells were allowed to migrate for 90 min. Leukocytes in the migrated and input wells were quantified using a fixed number of 15 μm polystyrene beads as a standard (Polyscience Inc.) combined with flow cytometric analysis of γδ, CD4 + and (γδ TCR − ) CD8 + T cells. ..

    Article Title: The skin, a novel niche for recirculating B cells
    Article Snippet: .. Recombinant mouse CCL17 and recombinant human CCL1, CCL20, and CCL28 (R&D Systems) were titrated in triplicates between 1 and 100 nM or 10 and 300 nM, and cells were allowed to migrate for 90 min. ELISA and ELISPOT assay For ELISAs, Immulon 4 HBX 96-well plates (Nunc) were coated overnight at 4°C with 1 μg/ml rabbit anti-ovine IgG (H+L) (Invitrogen), blocked with heat-inactivated rabbit serum (Gibco), and washed with PBS-Tween (PBS containing 0.05% Tween-20). ..

    Article Title: Chemokine (C-C Motif) Ligand 1 Derived from Tumor-Associated Macrophages Contributes to Esophageal Squamous Cell Carcinoma Progression via CCR8-Mediated Akt/Proline-Rich Akt Substrate of 40 kDa/Mammalian Target of Rapamycin Pathway.
    Article Snippet: .. They were treated with 0 or 10 ng/mL recombinant human CCL1 (rhCCL1; R&D Systems). .. After 0, 24, 48, or 96 hours, CellTiter 96 Aqueous One Solution Reagent (Promega, Madison, WI) was added.

    Concentration Assay:

    Article Title: STARTRAC analyses of scRNAseq data from tumor models reveal T cell dynamics and therapeutic targets
    Article Snippet: .. Recombinant human CCL1 (R&D Systems) was prepared at suboptimal concentration of 100 pM and added to the bottom Transwell chambers at 100 μl per well. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: The skin, a novel niche for recirculating B cells
    Article Snippet: .. Recombinant mouse CCL17 and recombinant human CCL1, CCL20, and CCL28 (R&D Systems) were titrated in triplicates between 1 and 100 nM or 10 and 300 nM, and cells were allowed to migrate for 90 min. ELISA and ELISPOT assay For ELISAs, Immulon 4 HBX 96-well plates (Nunc) were coated overnight at 4°C with 1 μg/ml rabbit anti-ovine IgG (H+L) (Invitrogen), blocked with heat-inactivated rabbit serum (Gibco), and washed with PBS-Tween (PBS containing 0.05% Tween-20). ..

    Enzyme-linked Immunospot:

    Article Title: The skin, a novel niche for recirculating B cells
    Article Snippet: .. Recombinant mouse CCL17 and recombinant human CCL1, CCL20, and CCL28 (R&D Systems) were titrated in triplicates between 1 and 100 nM or 10 and 300 nM, and cells were allowed to migrate for 90 min. ELISA and ELISPOT assay For ELISAs, Immulon 4 HBX 96-well plates (Nunc) were coated overnight at 4°C with 1 μg/ml rabbit anti-ovine IgG (H+L) (Invitrogen), blocked with heat-inactivated rabbit serum (Gibco), and washed with PBS-Tween (PBS containing 0.05% Tween-20). ..



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    Ccl1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Total RNA was extracted and trimmed from formalin‐fixed, paraffin‐embedded (FFPE) sections of three cases of CRC and CRLM (A, dotted line) and subjected to RNA sequencing. The top five genes related to the decreased expression of TAMs in CRLM compared with that in CRC are listed as targeted genes. The data are normalized by TPM (B). RNA scope® assay displays <t>CCL1</t> mRNA in the FFPE tissues from CRC and CRLM. The housekeeping gene PPIB stained positive in CRC and CRLM (C [a, b]). Little CCL1 mRNA expression is detected in the stroma of CRC (C [c], black arrowheads), while none is detected in that of CRLM (C [d]).
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    Total RNA was extracted and trimmed from formalin‐fixed, paraffin‐embedded (FFPE) sections of three cases of CRC and CRLM (A, dotted line) and subjected to RNA sequencing. The top five genes related to the decreased expression of TAMs in CRLM compared with that in CRC are listed as targeted genes. The data are normalized by TPM (B). RNA scope® assay displays <t>CCL1</t> mRNA in the FFPE tissues from CRC and CRLM. The housekeeping gene PPIB stained positive in CRC and CRLM (C [a, b]). Little CCL1 mRNA expression is detected in the stroma of CRC (C [c], black arrowheads), while none is detected in that of CRLM (C [d]).
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    Fig. 5 CCR8 on TITR mimics is functional in a Transwell migration assay. a Representative flow cytometry dot plot showing CCR8 and CCR4 expression on TITRs mimics, stained by BV421-CCR8 (BD, #566,379) and APC-CCR4 (Biolegend, #359,408). b Percentage of TITR mimics expressing CCR8 and CCR4 (n = 2). c Migration of TITR mimics toward <t>CCL1</t> and CCL22 in the presence or absence of NS-15 (n = 2). One-way ANOVA followed by Tukey test was per- formed to compare each condition with every other condition. The differences between selected pairs were shown, * p ≤ 0.05
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    Fig. 5 CCR8 on TITR mimics is functional in a Transwell migration assay. a Representative flow cytometry dot plot showing CCR8 and CCR4 expression on TITRs mimics, stained by BV421-CCR8 (BD, #566,379) and APC-CCR4 (Biolegend, #359,408). b Percentage of TITR mimics expressing CCR8 and CCR4 (n = 2). c Migration of TITR mimics toward <t>CCL1</t> and CCL22 in the presence or absence of NS-15 (n = 2). One-way ANOVA followed by Tukey test was per- formed to compare each condition with every other condition. The differences between selected pairs were shown, * p ≤ 0.05
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    Fig. 5 CCR8 on TITR mimics is functional in a Transwell migration assay. a Representative flow cytometry dot plot showing CCR8 and CCR4 expression on TITRs mimics, stained by BV421-CCR8 (BD, #566,379) and APC-CCR4 (Biolegend, #359,408). b Percentage of TITR mimics expressing CCR8 and CCR4 (n = 2). c Migration of TITR mimics toward <t>CCL1</t> and CCL22 in the presence or absence of NS-15 (n = 2). One-way ANOVA followed by Tukey test was per- formed to compare each condition with every other condition. The differences between selected pairs were shown, * p ≤ 0.05
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    Image Search Results


    Total RNA was extracted and trimmed from formalin‐fixed, paraffin‐embedded (FFPE) sections of three cases of CRC and CRLM (A, dotted line) and subjected to RNA sequencing. The top five genes related to the decreased expression of TAMs in CRLM compared with that in CRC are listed as targeted genes. The data are normalized by TPM (B). RNA scope® assay displays CCL1 mRNA in the FFPE tissues from CRC and CRLM. The housekeeping gene PPIB stained positive in CRC and CRLM (C [a, b]). Little CCL1 mRNA expression is detected in the stroma of CRC (C [c], black arrowheads), while none is detected in that of CRLM (C [d]).

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Reduced chemokine C‐C motif ligand 1 expression may negatively regulate colorectal cancer progression at liver metastatic sites

    doi: 10.1111/jcmm.18193

    Figure Lengend Snippet: Total RNA was extracted and trimmed from formalin‐fixed, paraffin‐embedded (FFPE) sections of three cases of CRC and CRLM (A, dotted line) and subjected to RNA sequencing. The top five genes related to the decreased expression of TAMs in CRLM compared with that in CRC are listed as targeted genes. The data are normalized by TPM (B). RNA scope® assay displays CCL1 mRNA in the FFPE tissues from CRC and CRLM. The housekeeping gene PPIB stained positive in CRC and CRLM (C [a, b]). Little CCL1 mRNA expression is detected in the stroma of CRC (C [c], black arrowheads), while none is detected in that of CRLM (C [d]).

    Article Snippet: Recombinant Human CCL1 Protein (rCCL1) was purchased from R&D Systems (cat. 272‐I‐010, Minneapolis, NE, USA).

    Techniques: Formalin-fixed Paraffin-Embedded, RNA Sequencing, Expressing, RNAscope, Staining

    Immunohistochemical expression of CCL1 and CCR8 in 16 cases of CRC and CRLM (A) and quantification by the manual‐visual counting method (B). CCL1‐positive cells are accumulated mainly in the stroma of CRC (A [a,b]), while few low‐intensity CCL1‐positive cells are observed in the stroma of CRLM (A [c,d]). A significant difference is observed in the quantity of CCL‐1 positive cells between CRC and CRLM. A significant difference is observed in the distribution of CCR8‐positive cells in CRC and CRLM (A [e–h]). Double immunofluorescence staining of CCL1 (green) and CD68 (red) with DAPI (blue) in CRC and CRLM (C). CD68 and CCL1 immunoreactivity is observed more in CRC than in CRLM (C [a–d], D [a, b]). CCL1 immunoreactivity is colocalized mostly with CD68 in CRC (C [g]), but rarely in CRLM (C [h]). The percentage of CCL1 in CD68 positive cells (CCL1/CD68) was significantly higher in CRC than in CRLM (D [c]). * p < 0.05.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Reduced chemokine C‐C motif ligand 1 expression may negatively regulate colorectal cancer progression at liver metastatic sites

    doi: 10.1111/jcmm.18193

    Figure Lengend Snippet: Immunohistochemical expression of CCL1 and CCR8 in 16 cases of CRC and CRLM (A) and quantification by the manual‐visual counting method (B). CCL1‐positive cells are accumulated mainly in the stroma of CRC (A [a,b]), while few low‐intensity CCL1‐positive cells are observed in the stroma of CRLM (A [c,d]). A significant difference is observed in the quantity of CCL‐1 positive cells between CRC and CRLM. A significant difference is observed in the distribution of CCR8‐positive cells in CRC and CRLM (A [e–h]). Double immunofluorescence staining of CCL1 (green) and CD68 (red) with DAPI (blue) in CRC and CRLM (C). CD68 and CCL1 immunoreactivity is observed more in CRC than in CRLM (C [a–d], D [a, b]). CCL1 immunoreactivity is colocalized mostly with CD68 in CRC (C [g]), but rarely in CRLM (C [h]). The percentage of CCL1 in CD68 positive cells (CCL1/CD68) was significantly higher in CRC than in CRLM (D [c]). * p < 0.05.

    Article Snippet: Recombinant Human CCL1 Protein (rCCL1) was purchased from R&D Systems (cat. 272‐I‐010, Minneapolis, NE, USA).

    Techniques: Immunohistochemical staining, Expressing, Double Immunofluorescence Staining

    Western blotting detected the levels of phosphorylated AKT (p‐AKT), total AKT, phosphorylated ERK (p‐ERK) and total ERK in Colo320DM cells treated with rCCL1 (10 ng/mL) for 0, 5, 10, 30, 60 min (A). p‐AKT/AKT expression in Colo320DM cells peaked at 30 min after rCCL1 treatment, whereas no expression of p‐ERK/ERK after rCCL1 treatment is observed (B). Inhibition of AKT phosphorylation in Colo320DM cells by treatment with 0.1, 0.5, 1.0 and 10 μM MK‐2206 dihydrochloride for 24 h is detected by Western blotting. Reduced p‐AKT level is observed by treatment with 10 μM MK‐2206 dihydrochloride (C). * p < 0.05.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Reduced chemokine C‐C motif ligand 1 expression may negatively regulate colorectal cancer progression at liver metastatic sites

    doi: 10.1111/jcmm.18193

    Figure Lengend Snippet: Western blotting detected the levels of phosphorylated AKT (p‐AKT), total AKT, phosphorylated ERK (p‐ERK) and total ERK in Colo320DM cells treated with rCCL1 (10 ng/mL) for 0, 5, 10, 30, 60 min (A). p‐AKT/AKT expression in Colo320DM cells peaked at 30 min after rCCL1 treatment, whereas no expression of p‐ERK/ERK after rCCL1 treatment is observed (B). Inhibition of AKT phosphorylation in Colo320DM cells by treatment with 0.1, 0.5, 1.0 and 10 μM MK‐2206 dihydrochloride for 24 h is detected by Western blotting. Reduced p‐AKT level is observed by treatment with 10 μM MK‐2206 dihydrochloride (C). * p < 0.05.

    Article Snippet: Recombinant Human CCL1 Protein (rCCL1) was purchased from R&D Systems (cat. 272‐I‐010, Minneapolis, NE, USA).

    Techniques: Western Blot, Expressing, Inhibition, Phospho-proteomics

    mRNA expression levels of CCR8, MMP‐2, MMP‐9, CDH1 and VEGFA in Colo320DM cells transfected with Ns siRNA or siRNA measured by RT‐qPCR. Transfection efficiency of CCR8 in Colo320DM cells measured by RT‐qPCR. Ns siRNA and siRNA were transfected into Colo320DM cells using Lipofectamine® 3000. Relative expression of CCR8 mRNA is significantly knocked down in Colo320DM cells transfected with siRNA (A). Expression of MMP‐2 in Colo320DM transfected with Ns siRNA plus rCCL1 (10 ng/mL) is significantly higher than transfection with siRNA plus rCCL1 (B). The data are normalized to internal control GAPDH. * p < 0.05.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Reduced chemokine C‐C motif ligand 1 expression may negatively regulate colorectal cancer progression at liver metastatic sites

    doi: 10.1111/jcmm.18193

    Figure Lengend Snippet: mRNA expression levels of CCR8, MMP‐2, MMP‐9, CDH1 and VEGFA in Colo320DM cells transfected with Ns siRNA or siRNA measured by RT‐qPCR. Transfection efficiency of CCR8 in Colo320DM cells measured by RT‐qPCR. Ns siRNA and siRNA were transfected into Colo320DM cells using Lipofectamine® 3000. Relative expression of CCR8 mRNA is significantly knocked down in Colo320DM cells transfected with siRNA (A). Expression of MMP‐2 in Colo320DM transfected with Ns siRNA plus rCCL1 (10 ng/mL) is significantly higher than transfection with siRNA plus rCCL1 (B). The data are normalized to internal control GAPDH. * p < 0.05.

    Article Snippet: Recombinant Human CCL1 Protein (rCCL1) was purchased from R&D Systems (cat. 272‐I‐010, Minneapolis, NE, USA).

    Techniques: Expressing, Transfection, Quantitative RT-PCR, Control

    The scratch test demonstrates a significant difference of cell motility between Colo320DM cells transfected with rCCL1 and Ns siRNA than with rCCL1 and siRNA. Representative time‐lapse images of scratch test (A). Each image was taken immediately and 60 h after the scratch assay. Quantitative analysis was carried out with IncuCyte ZOOM (B). Views of the scratch test after treatment with DMSO and MK‐2206 dihydrochloride (C). 10 μM MK‐2206 dihydrochloride markedly reduced rCCL1‐dependent cell motility in Colo320DM cells (D). * p < 0.05.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Reduced chemokine C‐C motif ligand 1 expression may negatively regulate colorectal cancer progression at liver metastatic sites

    doi: 10.1111/jcmm.18193

    Figure Lengend Snippet: The scratch test demonstrates a significant difference of cell motility between Colo320DM cells transfected with rCCL1 and Ns siRNA than with rCCL1 and siRNA. Representative time‐lapse images of scratch test (A). Each image was taken immediately and 60 h after the scratch assay. Quantitative analysis was carried out with IncuCyte ZOOM (B). Views of the scratch test after treatment with DMSO and MK‐2206 dihydrochloride (C). 10 μM MK‐2206 dihydrochloride markedly reduced rCCL1‐dependent cell motility in Colo320DM cells (D). * p < 0.05.

    Article Snippet: Recombinant Human CCL1 Protein (rCCL1) was purchased from R&D Systems (cat. 272‐I‐010, Minneapolis, NE, USA).

    Techniques: Transfection, Wound Healing Assay

    Fig. 5 CCR8 on TITR mimics is functional in a Transwell migration assay. a Representative flow cytometry dot plot showing CCR8 and CCR4 expression on TITRs mimics, stained by BV421-CCR8 (BD, #566,379) and APC-CCR4 (Biolegend, #359,408). b Percentage of TITR mimics expressing CCR8 and CCR4 (n = 2). c Migration of TITR mimics toward CCL1 and CCL22 in the presence or absence of NS-15 (n = 2). One-way ANOVA followed by Tukey test was per- formed to compare each condition with every other condition. The differences between selected pairs were shown, * p ≤ 0.05

    Journal: Cancer immunology, immunotherapy : CII

    Article Title: Development of a cellular model to study CCR8 signaling in tumor-infiltrating regulatory T cells.

    doi: 10.1007/s00262-023-03607-z

    Figure Lengend Snippet: Fig. 5 CCR8 on TITR mimics is functional in a Transwell migration assay. a Representative flow cytometry dot plot showing CCR8 and CCR4 expression on TITRs mimics, stained by BV421-CCR8 (BD, #566,379) and APC-CCR4 (Biolegend, #359,408). b Percentage of TITR mimics expressing CCR8 and CCR4 (n = 2). c Migration of TITR mimics toward CCL1 and CCL22 in the presence or absence of NS-15 (n = 2). One-way ANOVA followed by Tukey test was per- formed to compare each condition with every other condition. The differences between selected pairs were shown, * p ≤ 0.05

    Article Snippet: Recombinant human interleukin-2 (IL-2; #202-IL) and chemokine CCL1 (#272-I) were obtained from R&D systems.

    Techniques: Functional Assay, Transwell Migration Assay, Flow Cytometry, Expressing, Staining, Migration